Add Proteomic Analysis Of Lysine Acetylation Websites In Rat Tissues Reveals Organ Specificity And Subcellular Patterns

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 NOTE: After the coupling of the primary C-terminal amino acid of all peptides to the amino group of the membrane, the amino teams between the spots and also among the amino groups inside the spot areas don't form bonds with the amino acids. Be sure that all the purification buffers are at four °C and perform the protein purification in the chilly room to avoid protein denaturation. BE AWARE: This avoids the coupling of amino acids within the additional cycles with the membrane instead of the growing peptide chain.
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